Table 2.
Proteins identified from BioID proteomics analysis
| Expt 1 |
Expt 2 |
Expt 3 |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Protein | Gene | Score | Cov | Pep | Score | Cov | Pep | Score | Cov | Pep | |
| Interactome 1 | BAG family molecular chaperone regulator 2 | BAG2 | 17.93 | 24.17 | 4 | 7.26 | 18.48 | 3 | 20.17 | 27.96 | 4 |
| Coatomer subunit α | COPA | 14.05 | 5.15 | 6 | 1.76 | 0.90 | 1 | 9.88 | 4.98 | 5 | |
| DnaJ homolog subfamily B member 1 | DNAJB1 | 8.46 | 12.94 | 4 | 12.33 | 10.88 | 3 | 6.76 | 5.31 | 1 | |
| DnaJ homolog subfamily C member 10 | DNAJC10 | 5.19 | 10.41 | 4 | 9.53 | 6.31 | 4 | 10.95 | 9.58 | 7 | |
| Heat shock 105 kDa/110 kDa protein 1 | HSPH1 | 10.48 | 8.23 | 5 | 11.24 | 4.42 | 3 | 21.21 | 12.29 | 7 | |
| Hsc70-interacting protein | ST13 | 13.39 | 25.34 | 3 | 0.00 | 4.79 | 1 | 13.60 | 27.40 | 3 | |
| Protein disulfide-isomerase A3 | PDIA3 | 6.46 | 8.96 | 4 | 22.34 | 17.29 | 6 | 5.81 | 4.79 | 2 | |
| Serine/threonine-protein kinase ULK1 | ULK1 | 1.97 | 0.67 | 1 | 7.68 | 0.67 | 1 | 3.87 | 0.67 | 1 | |
| Interactome 2 | 40S ribosomal protein S10 | RPS10 | 51.73 | 5.45 | 1 | 43.93 | 5.45 | 1 | 49.81 | 5.45 | 1 |
| 60S ribosomal protein L3 | RPL3 | 35.98 | 6.84 | 1 | 57.51 | 1.74 | 1 | 28.28 | 16.52 | 1 | |
| DnaJ homolog subfamily B member 1 | DNAJB1 | 109.8 | 10.59 | 4 | 112.7 | 14.12 | 4 | 20.28 | 2.06 | 1 | |
| Heat shock protein HSP 90-α | HSP90AA1 | 241.7 | 17.21 | 5 | 302.7 | 13.8 | 5 | 182.9 | 12.16 | 2 | |
| Hemoglobin subunit α | HBA1 | 38.16 | 10.56 | 1 | 34.68 | 10.56 | 1 | 32.7 | 10.56 | 1 | |
| Integrin β1 | ITGB1 | 48.31 | 1.25 | 1 | 46.68 | 1.25 | 1 | 60.59 | 5.14 | 3 | |
| Nuclear migration protein nudC | NUDC | 154.8 | 21.75 | 6 | 133.3 | 22.66 | 7 | 99.66 | 16.31 | 5 | |
| Protein disulfide-isomerase A3 | PDIA3 | 62.53 | 4.16 | 2 | 60.98 | 6.53 | 3 | 51.57 | 8.71 | 4 | |
| Sorting nexin-1 | SNX1 | 103.6 | 10.34 | 5 | 93.28 | 7.47 | 4 | 50.46 | 1.92 | 1 | |
| Suppressor of G2 allele of SKP1 homolog | SUGT1 | 119.1 | 15.62 | 4 | 107 | 15.89 | 4 | 46.25 | 8.22 | 2 | |
| T-complex protein 1 subunit epsilon | CCT5 | 44.94 | 6.47 | 2 | 74.54 | 7.21 | 4 | 107.3 | 10.54 | 5 | |
| T-complex protein 1 subunit eta | CCT7 | 102 | 5.52 | 3 | 60.97 | 4.42 | 2 | 120.1 | 15.65 | 7 | |
| Transgelin-2 | TAGLN2 | 67.72 | 11.56 | 2 | 65.84 | 23.62 | 4 | 115.6 | 16.58 | 3 | |
Score is sum of the ion scores for each unique peptide using Mascot software. Proteins identified by both experimental designs are bolded. Cov, percentage of the protein sequence covered by identified peptides; Pep, unique peptides.