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. Author manuscript; available in PMC: 2023 Apr 1.
Published in final edited form as: Biomaterials. 2022 Feb 23;283:121437. doi: 10.1016/j.biomaterials.2022.121437

Figure 4.

Figure 4.

Attenuation of oxidative damage by SOD-NPs in mouse SFs and human OA synovial explants. (A) Representative fluorescence images of mouse SFs treated with SOD(FITC)-NP(Rhod) for 24 h. Scale bar: 50 μm. (B) Measurement of H2DCFDA levels in mouse SFs after being treated by TNFα plus PBS, empty NP, SOD, or SOD-NP for 24 h. (C) Representative immunohistochemistry images of 8-OHdG in human synovial explants treated with PBS alone or IL-1β in combination with PBS, empty NP, SOD or SOD-NPs for 8 days. Scale bar: 100 μm. (D) The mean ratio of integrated optical density (IOD) to area (IOD/area) was used to semi-quantify 8-OHdG amount. (n = 5 explants/group). (E) Immunohistochemistry staining of Mmp13. Scale bar, 100 μm. (F) Semi-quantitative evaluation of Mmp13 amount represented as IOD/area. (n = 5 explants/group). (G) Immunohistochemistry staining of Adamts5. Scale bar: 100 μm. (H) Semi-quantitative evaluation of Adamts5 amount represented as IOD/area. (n = 5 explants/group). In panels C, E, and G, the bottom images are the magnified images from the squared regions from the top images. Low: low magnification; high: high magnification. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.