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. 2022 Apr 4;7:98. doi: 10.1038/s41392-022-00915-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Genome-wide CRISPR/Cas9 screening in Nalm6 cells cocultured with CD19 CAR T cells. a Schematic of the CRISPR/Cas9 screening. b Volcano plots of essential enriched sgRNAs after screening. c KEGG, GO, and Reactome pathway enrichment analysis of genes identified in CRISPR screening