TABLE 2.
Key reagents used in immunofluorescent protocol.
| Antibody (Cat no) | Dilution (antigen retrieval) | Origin | Blocking agent | Detection | Cell type detected |
|---|---|---|---|---|---|
| Primary antibodies | |||||
| SOX9 (AB5535) | 1:10,000 (none) | Rabbit | Normal goat serum/TBS/BSA | Tyramide-FITC | Sertoli cell |
| CC3 (#9661) | 1:100 (0.01 M citrate buffer) | Rabbit | Normal goat serum/TBS/BSA | Tyramide-Cy3 | Apoptotic cell |
| AMH (sc-6886) | 1: 1,000 (none) | Goat | Normal chicken serum/TBS/BSA | Tyramide- Cy5 | Sertoli cell |
| Secondary antibodies | |||||
| Anti-rabbit peroxidase (PI-1000) | 1:200 | Goat | |||
| Anti-goat peroxidase (sc-2961) | 1:200 | Chicken | |||