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. 2022 Apr 3;6(4):e395. doi: 10.1002/pld3.395

FIGURE 6.

FIGURE 6

Fatty acid composition and gene expression changes in developing seeds of seed‐specific overexpressed GUS and DPBF2 in WT background. (a) Vectors used for seed‐specific heterologous expression of GUS and seed‐specific overexpression of DPBF2. (b) Comparison of FA composition in seeds of three independent Ph‐GUS and Ph‐DPBF2 T2 generation transgenic plants. FA content represents the mean (±SE) from three independent biological replicates. (c) Comparison of DPBF2 and FA biosynthesis gene expression in S6 and S7 stage of developing siliques between Ph‐GUS and Ph‐DPBF2 transformed plants. RT‐qPCR analysis of DPBF2, FAD2, FAD3, LPCAT1/2, PDCT, and FAE1 in the Ph‐GUS and Ph‐DPBF2 overexpression lines containing the phaseolin promoter. The S6 and S7 siliques used in this study included seeds of the walking‐stick embryo and curled cotyledon phase, respectively. Statistically significant differences by unpaired t test are indicated (*p < .05, **p < .01, ***p < .001). Relative expression values are given in comparison with the WT (WT = 1). Mean (±SE) from three independent biological replicates