Figure 4.

PTPRM is critical for synapse formation regulated by Zn²⁺. (A) Representative images of neurons overexpressed PTPRM or not cultured with/without 50 μM Zn²⁺ when expressed PTPRM or not. Neurons were fixed and labeled by synapsin1 (to mark synapses) and Map AP2 (to mark dendrites) antibodies. The scale bar in the right lower corner applies to all images. (B) Summary graphs of synapse number calculated from the number of synapsin1-specific puncta for the conditions described in (A). (C) Summary graphs of synapse size calculated from the area of synapsin1-specific puncta for the conditions described in (A). Data information: numbers of cells/independent cultures analyzed are listed in the bars. Data shown in summary graphs are mean values ± SEM. Statistical significance was analyzed by Student’s t-test. **p < 0.01. Scale bar: 5 μm.