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. 2022 Mar 21;9:832713. doi: 10.3389/fmed.2022.832713

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Copyright © 2022 Wu, Hsu, Hu, Lan, Yeh and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Transduction of the PEP-NASP into RAW 264.7 cells. Cells were cultured in 60-mm culture dishes. Then, PEP-NASP (0.1 ~ 0.4 μM) and control NASP were added to the culture media for 1 h (A), and PEP-NASP (0.4 μM) and control NASP were added to the culture media for 10 ~ 60 min (B), and analyzed by immunoblotting (IB) with an antibody against His or β-actin. Transduction of PEP-NASP stability was assessed after various time periods (1 ~ 60 h). Cells pretreated with 0.4 μM PEP-NASP for 1 h and analyzed by IB with an antibody against His or β-actin (C). The intracellular distribution of the transduced PEP-NASP and NASP was observed by confocal microscopy (D). Scale bar = 25 μm. Data represent a minimum of three independent experiments.