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. 2022 Mar 21;9:832713. doi: 10.3389/fmed.2022.832713

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Copyright © 2022 Wu, Hsu, Hu, Lan, Yeh and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The PEP-NASP inhibited the infiltration of inflammatory cells in LPS-induced acute lung injury. (A) Total infiltrated cells in bronchoalveolar lavage fluid (BALF) were counted using a counting chamber. (B) Effect of the PEP-NASP on myeloperoxidase (MPO) activity in lung tissue homogenates. (C,D) Statistical analysis of percentages of neutrophils and macrophages in BALF by hemocytometer and Wright-Giemsa staining. (E) Production of ROS and (F) GSH level were measured in BALF. Values represent the mean ± standard error of the mean (SEM), n = 10, **p < 0.01 (by a one-way ANOVA).