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Correction of TO-PRO-3 fluorescence for variation in bacterial size. (a) Black line, S. aureus control cells; gray line, control cells permeabilized by heat treatment (10 min, 100°C). (b) S. aureus treated with 0.5 μg of amoxicillin per ml for 180 min. TO-PRO-3 fluorescence is uncorrected. (c) Same data as in panel b, but with TO-PRO-3 fluorescence corrected as described in the Results section.
