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. 2022 Feb 17;13(2):1403–1417. doi: 10.1002/jcsm.12938

Figure 1.

Figure 1

Patients with osteoporotic fracture (OPF) and having reduced proliferation of human skeletal muscle satellite cells (hSMSCs) and weakened osteogenic differentiation. (A) Double fluorescent immunostaining of Pax7 and BrdU in hSMSCs. (B) Five fields were randomly selected from each specimen under a microscope. The double staining positive cells were counted in the visual field. *P < 0.05, the positive expression of the normal fracture group was statistically different from that of the OPF group. The OPF group was significantly less than the normal fracture group. (C) ALP staining of hSMSCs in patients with non‐OPF was more obvious than that of patients with OPF. (D) *P < 0.05, ALP was significantly reduced in the OPF group compared with the non‐OPF group. The results of RT‐PCR of hSMSCs related genes in patients with normal fracture and OPF. (E) *P < 0.05, significant expression of BMP2 in normal fracture group compared with OPF group. (F) *P < 0.05, there was a statistically significant difference in Runx2 between the OPF group and the normal fracture group. (G) Double immunofluorescence staining of β‐catenin + Scal1 in hSMSC. (H) ***P < 0.001, compared with the normal fracture group, the β‐catenin + Scal1 double immunofluorescence staining in the OPF group was statistically significantly reduced.