Figure 8.

P7C3 treatment improves the Nampt enzymatic activity. (A) Enzymatic activity of recombinant Nampt was measured every 5 min for 1 h. Negative control (DMSO), positive control (Nampt enzyme), Nampt inhibitor (FK866), and Nampt activator (P7C3) were all utilized to assess the enzymatic activity. (B) The gastrocnemius muscle Nampt enzymatic activity was measured every 5 min for a total duration of 40 min. Data are expressed as mean ± SEM; *P < 0.05 of the absorbance measured at 20 min during the linear range. (C) Serum pyruvate activity of db‐Veh and db‐P7C3 treated mice. Data expressed as mean ± SEM with P < 0.05.