Figure 1.
Decrease in m6A levels in denervated muscles is accompanied by upregulation of ALKBH5. (A) m6A mRNA levels in gastrocnemius (GAS) muscles on Days 7, 14, and 28 post‐denervation as determined using liquid chromatography–tandem mass spectrometry (LC–MS/MS) (up) and dot‐blot (down) (n = 5). (B and C) METTL3, METTL14, and FTO protein levels in innervated and denervated GAS muscles (n = 5). GAPDH was used as the internal reference. (D and E) expression and quantification of ALKBH5 and FoxO3 protein in GAS muscles at 7, 14, and 28 days post‐denervation (n = 5). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, n.s., no significant by two‐tailed Student's t‐test.