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. 2022 Feb 22;13(2):1164–1176. doi: 10.1002/jcsm.12949

Figure 6.

Figure 6

rIL‐6‐treated endurance‐trained mice show increased expression of mitochondrial complex I components and PDK4. Relative M. quadriceps femoris mRNA levels of (A) mitochondrial genes of the electron transport chain [complex (C) I–V, Cyc] and tricarboxylic acid (TCA) cycle and (B) of pyruvate dehydrogenase kinase 4 (Pdk4), pyruvate dehydrogenase E1 component subunit alpha (Pdha1), and pyruvate dehydrogenase phosphatase 1 (Pdp1). Sed+Saline, n = 9; Sed+IL‐6, n = 9; Ex+Saline, n = 7; and Ex+IL‐6, n = 11. Expression values were determined by qPCR and normalized to Tbp. Data are shown as the mean fold change ± SEM including individual values relative to the expression of the control (Sed+Saline) set to 1. Relative M. quadriceps femoris protein levels of OXPHOS (C), NDUFB5 (D), and PDK4 (E) assessed by western blot (n = 6 per group). Target band intensities were normalized to the loading control (eEF2, α‐actinin, or Ponceau S stain), and data are shown as the mean fold change ± SEM including individual values relative to the control (Sed+Saline) set to 1. One‐way ANOVA followed by Sidak's multiple comparisons (A–E). *P < 0.05, **P < 0.01, and ***P < 0.001.