TABLE 3.
Intracellular concentrations of β-l-ddA and metabolites in human primary cultured hepatocytes and percent total radioactivity in each metabolite after incubation with 10 μM [2′,3′,8-3H]β-l-ddA for specific time periods
| Compound | Concna
|
|||
|---|---|---|---|---|
| 2 h | 4 h | 8 h | 24 h | |
| β-l-ddA | 29.05 ± 7.34 (58.6) | 25.36 ± 3.34 (54.4) | 22.98 ± 2.65 (52.8) | 24.07 ± 1.55 (53.5) |
| β-l-ddAMP | ND | 0.41 ± 0.07 (0.9) | 0.75 ± 0.12 (1.7) | 1.12 ± 0.13 (1.8) |
| β-l-ddADP | ND | ND | 0.92 ± 0.42 (2.1) | 0.81 ± 0.41 (1.3) |
| β-l-ddATP | ND | ND | ND | ND |
| Ribonolactone | 0.24 ± 0.10 (0.5) | 0.29 ± 0.12 (0.6) | ND | ND |
| Hypoxanthine | 0.43 ± 0.20 (0.9) | 0.69 ± 0.31 (1.5) | 0.52 ± 0.16 (1.2) | 0.27 ± 0.07 (0.6) |
| Inosine | 1.90 ± 0.81 (3.8) | 2.68 ± 0.59 (5.7) | 3.90 ± 1.97 (8.9) | 4.60 ± 2.57 (10.2) |
| ADP | 1.73 ± 0.45 (3.5) | 1.98 ± 0.32 (4.2) | 2.19 ± 0.61 (5.0) | 2.16 ± 0.53 (4.8) |
| ATP | 15.60 ± 1.74 (31.5) | 13.99 ± 1.43 (30.0) | 10.99 ± 1.30 (25.2) | 9.38 ± 1.19 (20.8) |
| β-l-ddA-Gluc | 0.61 ± 0.34 (1.2) | 1.25 ± 0.47 (2.7) | 1.31 ± 0.29 (3.0) | 2.60 ± 0.36 (5.8) |
a
Picomoles/106 cells ± standard deviation of three replications; percent total radioactivity is shown in parentheses. ND, not detectable.