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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Plant J. 2021 Dec 18;109(5):1229–1248. doi: 10.1111/tpj.15629

Figure 4.

Figure 4.

TOR inhibition rescues the Ire1a Ire1b root growth phenotype. (a) Representative images of WT and Ire1a Ire1b grown for 7, 10, or 12 days on plates containing 150 nM AZD-8055 or DMSO control. Red lines represent approximate average root length of the displayed seedlings. (b) Shoot fresh weight was determined by averaging WT or Ire1a Ire1b shoots grown in an individual plate for each plate replicate (n = 10; error bars show the standard deviation). (c) The length of individual roots was measured using ImageJ. For Figure 1(b, c): error bars show the standard deviation; significance markers displayed above an Ire1a Ire1b experimental group are pairwise comparisons to the corresponding WT group for that specific treatment. Brackets denote other specific pairwise comparisons. Significance markers: NS = Padj > 0.01; *0.001 < Padj < 0.01; **0.0001 < Padj < 0.001; ***Padj < 0.0001. (d) The angle of the root tip with the vertical axis (0°) was measured using ImageJ. Significance differences between coefficients of variation were tested using the asymptotic Feltz and Miller test as described in the Experimental Procedures section. Significance markers: NS = P > 0.0001; **P < 0.0001 and P > 1.0 × 10−10; ***P < 1.0 × 10−10.