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. Author manuscript; available in PMC: 2023 Mar 1.
Published in final edited form as: Plant J. 2021 Dec 18;109(5):1229–1248. doi: 10.1111/tpj.15629

Figure 5.

Figure 5.

TOR is hyperactive in Ire1a Ire1b mutant root tips but not in the mature root. (a) Representative immunoblot assay to determine relative S6K phosphorylation levels. Antibodies against total S6K (αS6K1/2) or phosphorylated S6K (Phos-S6K) were used against total soluble protein extracted from excised WT or Ire1a Ire1b root tips grown for 7 days on media containing AZD-8055 or DMSO control (see Experimental Procedures). Relative signals (Phos-S6K/αS6K1/2) in each experimental group were normalized to WT DMSO control (n = 11). (b) Same immunoblot method used in (a) but used against total soluble protein extracted from excised mature WT or Ire1a Ire1b root tissues grown for 7 days on media containing AZD-8055 or DMSO control (n = 9). For all graphs error bars show the standard deviation; significance markers displayed above an Ire1a Ire1b experimental group are pairwise comparisons to the corresponding WT group for that specific treatment. Brackets denote other specific pairwise comparisons. Significance markers: NS = Padj > 0.05; *0.005 < Padj < 0.05; **0.0005 < Padj < 0.005; ***Padj < 0.0005. See Figure S9 for full blot images and Ponceau stain loading controls.