Figure 1.

Elaiophylin inhibits cell proliferation and induces autophagic cell death in C918 cells. (A) The chemical structure of Elaiophylin; (B) C918, OCM1A and Mel270 cells were treated with different concentrations of Elaiophylin (0, 0.5, 1 and 2 μM) for 24 h. Cell viability was determined by MTT assay. (C) Five primary UM cells were treated with different concentrations of Elaiophylin (0, 0.5, 1 and 2 μM) for 24 h. Cell viability was determined by MTT assay. (D) C918 and ARPE-19 cells were treated with different concentrations of Elaiophylin (ranged from 0 to 2 μM) for 24 h. Cell viability was determined by MTT assay. (E) C918 cells were treated with indicated concentrations of Elaiophylin for 24h, and then apoptotic cells stained with Annexin V-FITC/PI were detected with flow cytometry. Apoptotic rates are shown in bars. Data was expressed as mean ± SD of three experiments and each experiment included triplicate repeats. ^* p<0.05, ^** p<0.01 vs. control.