Figure 2.

Elaiophylin induces oxidative stress and mitochondrial dysfunction in C918 cells. C918 cells were exposed to indicated concentrations of Elaiophylin, with or without NAC (10 μM) pre-treatment. (A, D) The representative images of ROS measurement (green fluorescence) in C918 cells after 2 h exposure to Elaiophylin. Intracellular ROS levels were detected by DCFH-DA fluorescent probe. The representative images of fluorescent probe were shown on the left and the quantitative analysis of ROS level was shown on the right. (B, E) The MMP of C918 cells exposed to indicated concentrations of Elaiophylin, with or without NAC. (C, F) Lysates of C918 cells exposed to indicated concentrations of Elaiophylin, with or without NAC were separated into cytoplasmic and mitochondrial fractions. Cytochrome c translocation was measured by western blotting. GAPDH and VDAC1 were used as loading controls for cytoplasm and mitochondria, respectively. Data was expressed as mean ± SD of three experiments. ^** p<0.01 vs. control.