FIG. 5.

Immunochemical detection of carbonyl groups in Achilles tendon collagen of mice after pefloxacin administration. A total of 10 μg of DNPH-derivatized protein was loaded onto each lane. (A) Lanes 1 to 3, Achilles tendon collagen of mice treated with pefloxacin (400 mg/kg/day) for 7 days; lanes 4 to 6, Achilles tendon collagen of control mice. (B) Densitometric analysis of the immunoblots in panel A. Values are means ± standard deviations. ∗, P < 0.05, and ∗∗, P < 0.01, versus controls (Student's t test).