FIG. 6.

Immunochemical detection of carbonyl groups in Achilles tendon collagen of rats after pefloxacin administration and ischemia (2 h) and reperfusion (3 days). A total of 15 μg of DNPH-derivatized protein was loaded onto each lane. (A) Lanes 1 and 2, Achilles tendon collagen of rats treated with pefloxacin (400 mg/kg/day) for 7 days; for this group I-R and sham surgery were done on the 4th day of pefloxacin treatment (duplicate loads); lanes 3 and 4, Achilles tendon collagen of rats that underwent sham surgery and that were treated with pefloxacin (400 mg/kg/day) for 7 days (duplicate loads); lanes 5 and 6, Achilles tendon collagen of rats submitted to a tendinous I-R (duplicated loads); lanes 7 and 8, Achilles tendon collagen of rats that underwent sham surgery and that received saline solution for 7 days. (B) Densitometric analysis of the immunoblots in panel A. Values are means ± standard deviations. ∗, P < 0.05, and ∗∗, P < 0.01, versus controls (Student's t test).