Table 2.

List of forward and reverse primers used for the bacterial quantification by QPCR

Target bacterial group	Forward primer 5′ to 3′ Reverse primer 5′ to 3’	Amplicon length, bp	Tm, °C	References
Salmonella enterica	F: TACTCAACATGGACGGCTCC R: TTTGCAAGAGAGAAGCGGGT	630	59.3 57.3	This study
Total bacteria	F: GTGCCAGCMGCCGCGGTAA R: GACTACCAGGGTATCTAAT	291	64.2 52.4	[47]
Lactobacillus spp.	F: AGCAGTAGGGAATCTTCCA R: CACCGCTACACATGGAG	341	54.5 55.2	[48]
Bifidobacterium spp.	F: GCGTGCTTAACACATGCAAGTC R: CACCCGTTTCCAGGAGCTATT	125	60.3 59.8	[49]
Enterobacteriaceae	F: ATGTTACAACCAAAGCGTACA R: TTACCYTGACGCTTAACTGC	185	54.0 56.3	[50]
Butyryl-CoA:acetate CoA-transferase (B-CoA)	F: GCIGAICATTTCACITGGAAYWSITGGCAYATG R CCTGCCTTTGCAATRTCIACRAANGC	530	67.0 64.0	[51]
Prevotella spp.	F: CACRGTAAACGATGGATGCC R: GGTCGGGTTGCAGACC	514	58.3 56.9	[52]

bp, Base pairs; Tm, Melting temperature