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. 2022 Apr 4;23:258. doi: 10.1186/s12864-022-08522-8

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — The effect of DYNLL2 and miR-148a-3p on CPMs differentiation. A Relative expression of DYNLL2 after overexpression in CPMs. B The changes of differentiation marker genes MYOD, MYOG, and MYHC after DYNLL2 transfection in CPMs. C MYHC staining after transfection of DYNLL2. D Changes in the differentiation marker MYHC protein after DYNLL2 transfection of CPMs (The order of the samples, lanes 1-2: pcDNA3.1, DYNLL2). E The relative expression of miR-148a-3p after transfection with miR-148a-3p mimics. F The changes of differentiation marker genes MYOD, MYOG, and MYHC after miR-148a-3p transfection in CPMs. G MYHC staining after transfection with the miR-148a-3p mimic in CPMs. H Changes in the differentiation marker MYHC protein after transfection with the miR-148a-3p mimics (The order of the samples, lanes 1-2: mimic NC, miR-148a-3p mimic). I Changes in the differentiation marker MYHC protein after co-transfection of miR-148a-3p mimics and DYNLL2. (The order of the samples, lanes 1-3: mimic NC, miR-148a-3p mimic, miR-148a-3p mimic+DYNLL2). The data are expressed as the mean ± S.E.M. (* p <0.05; ** p <0.01, *** p <0.001)