Fig. 3.

A subset of T2Rs localize to the nucleus of two HNSCC cell lines. Fixed cultures of HNSCC cell lines (A) SCC4 and (B) SCC47 stained with antibodies targeting endogenous proteins demonstrate nuclear localization of T2R42. T2R13 appears to localize strongly to the nucleus in SCC47 but not in SCC4. T2Rs 4 and 14 are expressed in both cell lines and appear to localize to the plasma membrane. For all images, 1 representative image from 3 experiments was shown. Scale bar represents 10 µm. Each antibody was compared to secondary only control at the same microscope settings.