Fig. 6.

LPS‐mediated activation of S100A7 counteracts TLR4 expression in breast cancer cells. Expression of (A) S100A7 and (B) TLR4 proteins was analyzed in MDA‐MB‐231 vector (231V)‐ and S100A7‐overexpressing MDA‐MB‐231 (S7OE) breast cancer cells by western blot analysis. (C) Expression of S100A7 was analyzed in S7OE breast cancer cells treated with LPS either in the presence or in absence of PMB for 24 h. (D) Expression of TLR4 in 231V and S7OE breast cancer cells was analyzed after treatment with PBS or LPS for 24 h by western blot. GAPDH was used as a loading control. Effect of LPS on (E) wound healing and (F) migrating abilities of 231V and S7OE breast cancer cells. The data presented here are the mean ± SEM of triplicate experiments (n = 3). One‐way ANOVA (Tukey's multiple comparisons test) was used to calculate the P‐values (*P < 0.05, **P < 0.01).