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. 2022 Mar 17;36(7-9):480–504. doi: 10.1089/ars.2021.0023

FIG. 5.

FIG. 5.

Analysis of miRNAs by qRT-PCR. (A) Differential expression of six miRNAs chosen from the RNA-seq experiment was confirmed by qRT-PCR. Data show mean ± SEM of abundance of each miRNA in circulating EVs obtained from mice undergoing BDL (different time points) and respective sham controls (n ≥ 4); *a4d BDL versus 21d sham, **a4d BDL versus 4d sham, 8d sham, and 15d sham, *b8d BDL versus 4d BDL, ***b8d BDL versus 4d sham, 8d sham, 15d sham, and 21d sham, *c15d BDL versus 4d sham, 8d sham, and 15d sham, ***c15d BDL versus 8d BDL, ***d21d BDL versus 8d BDL, ***e8d BDL versus 4d sham, 8d sham, 15d sham, 21d sham, and 8d BDL, ***f15d BDL versus 8d BDL, *g21d BDL versus 4d sham and 8d sham, **g21d BDL versus 15d sham, **h8d BDL versus 4d BDL, ***h8d BDL versus 4d sham, 8d sham, 15d sham, and 21d sham, ***i15d BDL versus 8d BDL, *j21d BDL versus 15d BDL, **j21d BDL versus 4d sham, 8d sham, 15d sham, and 21d sham, **k21d BDL versus 4d sham, 21d sham, 4d BDL, and 15d BDL, ***k21d BDL versus 8d sham and 15d sham, *l8d BDL versus 15d sham and 4d BDL, *m21d BDL versus 21d sham, **m21d BDL versus 4d sham, ***m21d BDL versus 8d sham, 15d sham, 4d BDL, and 15d BDL, *n21d BDL versus 15d sham, **n21d BDL versus 4d sham. (B) Data show mean ± SEM of abundance of each miRNA in the liver of mice undergoing BDL and respective sham controls (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001.