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. 2022 Mar 17;36(7-9):480–504. doi: 10.1089/ars.2021.0023

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© Sharmila Fagoonee et al., 2022; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 8. — Analysis of DDC-fed and Mdr2-/- cholestatic mice. (A) Collagen deposits (red) were analyzed by PSR staining in the livers of mice fed on DDC-diet for 1 and 2 weeks with respect to NT. (B) Biochemical parameters analysis in DDC-treated mice. (C) Fibrosis-related gene expression analysis DDC-treated mice (n = 6). (D) miRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the DDC-fed mice. Data show mean ± SEM of abundance of each miRNA in circulating EVs (n = 6). (E) Data show mean ± SEM of abundance of each miRNA in the liver of DDC-fed mice by qRT-PCR (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001. (F) Collagen deposits (red) were analyzed by PSR staining in the Mdr2-/- mice livers at 4 and 6 weeks of age with respect to WT controls (n = 12). (G) Biochemical parameters analysis in Mdr2-/- mice (n = 6). (H) Fibrosis-related gene expression analysis Mdr2-/- mice. (I) miRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the Mdr2-/- mice (n ≥ 5). Data show mean ± SEM of abundance of each miRNA in circulating EVs (n ≥ 4). (J) Data show mean ± SEM of abundance of each miRNA in the liver of Mdr2-/- mice by qRT-PCR (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001. (K) mRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the DDC-fed mice and Mdr2-/- mice, respectively. Data show mean ± SEM of abundance of Alas2 in circulating EVs (n = 3). (L) The expression of the mRNAs in the DDC-treated and Mdr2-/- mouse livers was also analyzed, and data show mean ± SEM (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001. NT, non-treated mice; WT, wild type. Color images are available online.

FIG. 8. — Analysis of DDC-fed and Mdr2-/- cholestatic mice. (A) Collagen deposits (red) were analyzed by PSR staining in the livers of mice fed on DDC-diet for 1 and 2 weeks with respect to NT. (B) Biochemical parameters analysis in DDC-treated mice. (C) Fibrosis-related gene expression analysis DDC-treated mice (n = 6). (D) miRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the DDC-fed mice. Data show mean ± SEM of abundance of each miRNA in circulating EVs (n = 6). (E) Data show mean ± SEM of abundance of each miRNA in the liver of DDC-fed mice by qRT-PCR (n = 6). *p < 0.05, **p < 0.01, ***p < 0.001. (F) Collagen deposits (red) were analyzed by PSR staining in the Mdr2-/- mice livers at 4 and 6 weeks of age with respect to WT controls (n = 12). (G) Biochemical parameters analysis in Mdr2-/- mice (n = 6). (H) Fibrosis-related gene expression analysis Mdr2-/- mice. (I) miRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the Mdr2-/- mice (n ≥ 5). Data show mean ± SEM of abundance of each miRNA in circulating EVs (n ≥ 4). (J) Data show mean ± SEM of abundance of each miRNA in the liver of Mdr2-/- mice by qRT-PCR (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001. (K) mRNAs identified as enriched in circulating by RNA-seq in the BDL model were analyzed by qRT-PCR in the DDC-fed mice and Mdr2-/- mice, respectively. Data show mean ± SEM of abundance of Alas2 in circulating EVs (n = 3). (L) The expression of the mRNAs in the DDC-treated and Mdr2-/- mouse livers was also analyzed, and data show mean ± SEM (n ≥ 3). *p < 0.05, **p < 0.01, ***p < 0.001. NT, non-treated mice; WT, wild type. Color images are available online.