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Quantification of fIHC. Using ImageJ, mean intensity (MI) per unit area in arbitrary units (a.u.) was assessed for fIHC labeling of GFAP, GS, and IBA1 (A); NF-κB, NLRP3, and TNFα (B); and STAT3, pSTAT3-Y705 and S727, and SOCS3 (C) in WT (black) and BXD32 (grey) mouse retinas with age. Error bars = ±SEM. *, p < 0.05; **, p < 0.005.
