FIGURE 3.

Silencing SIDT2 and RBBP8NL altered NHPK differentiation. NHPK cells were transfected with SIDT2 siRNA, RBBP8NL siRNA, and scrambled siRNA duplexes for 24 hours, and then differentiated in EpiLife with 1.3 mM CaCl₂ for 5 days. (A) Expression of FLG, LOR, IVL, and KRT10 in differentiated NHPK were evaluated by real-time qRT-PCR. Data of three independent experiments are shown. Data are presented as the geometric mean (95% CI) of relative gene expression. Comparisons of log-transformed relative gene expression values between target siRNA and scrambled siRNA were made using t tests. Significant differences had *p-value < 0.05 and ns = not significant. (B) Western blot assay to detect protein expression in differentiated NHPK; cell lysates were prepared from NHPK of three different donors. *Note: Hard edges/pixilation in panel 3B have been editorially validated in source images