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[Preprint]. 2022 Mar 29:2022.03.29.486282. [Version 1] doi: 10.1101/2022.03.29.486282

Figure 4:

Figure 4:

Effect of chronic intermittent ethanol (CIE) exposure on the 5HT, ACE2, and TMPRSS2 immunoreactivity in dorsal raphe nucleus (DRN). Representative confocal images showing (A and F) 5HT (blue) (B and G) ACE2 (red) (C and H) TMPRSS2 (green) (D and I) 5HT-ACE2 (magenta) (E and J) 5HT-TMPRSS2 (white) positive neurons in DRN of air and CIE mice. Scale bar = 100 μm. Representative single tile confocal images showing (K and L) 5HT (blue) (M and N) ACE2 (red) (O and P) TMPRSS2 (green) (Q and R) 5HT-ACE2 (magenta) (S and T) 5HT-TMPRSS2 (white) positive neurons in DRN of air control and CIE mice. Scale bar = 50 μm. Graph showing (U) % immunoreactive area for 5HT, ACE2, and TMPRSS2, (V) % immunoreactive area for 5HT-ACE2 positive neurons, (W) Pearson correlation coefficient for 5HT-ACE2 colocalization (X) Mander’s overlap coefficient for 5HT-ACE2 colocalization in DRN. Values (n = 4 – 5/group) are represented as means (±SEM) and the data was analyzed by unpaired student’s t-test with Welch’s correction (*p < 0.05 versus air control).