Figure 2.

Co-localization of stromal neutrophils and T cells associated with Overall survival. (A) Kaplan-Meier overall survival (OS) curves were designed according to high- and low-density of combined stromal CD66b⁺ neutrophil and CD4⁺ T cell infiltration or combined stromal CD66b⁺ neutrophil and CD8⁺ T cell infiltration. Patients with high levels of CD4⁺/CD8⁺ T cell infiltration alone had a significantly better OS than patients with high levels of combined CD4⁺/CD8⁺ T cell and neutrophil infiltration. P-values were determined using a log-rank test. ** P < 0.001; *** P < 0.0001. (B) Positive associations between the number of ${\mathrm{CD66}}_{\text{ST}}^{+}$ cells and ${\mathrm{CD4}}_{\text{ST}}^{+}$ cells, ${\mathrm{CD66}}_{\text{ST}}^{+}$ cells and ${\mathrm{CD8}}_{\text{ST}}^{+}$ cells (n = 237). (C) Representative pictures showing the co-localization of neutrophil (CD66b, green) and T cell markers (CD4 and CD8, red) in the stromal (ST) regions of UCB tissues. (D, E) Consecutive sections were used for the immunohistochemical identification of stromal CD66b⁺ cells, as well as CD4⁺ T cells and CD8⁺ T cells in UCB tissues (n= 57). P < 0.05 was considered significant difference (Spearman’s rank correlation coefficient test). Representative examples of tumors with either low (top) or high (bottom) expression of neutrophil (CD66b), T cells (CD4 and CD8) markers are shown. Scale bar: 100 μm.