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. 2022 Mar 21;13:827457. doi: 10.3389/fimmu.2022.827457

Figure 2.

Figure 2

Co-localization of stromal neutrophils and T cells associated with Overall survival. (A) Kaplan-Meier overall survival (OS) curves were designed according to high- and low-density of combined stromal CD66b+ neutrophil and CD4+ T cell infiltration or combined stromal CD66b+ neutrophil and CD8+ T cell infiltration. Patients with high levels of CD4+/CD8+ T cell infiltration alone had a significantly better OS than patients with high levels of combined CD4+/CD8+ T cell and neutrophil infiltration. P-values were determined using a log-rank test. ** P < 0.001; *** P < 0.0001. (B) Positive associations between the number of CD66ST+ cells and CD4ST+ cells, CD66ST+ cells and CD8ST+ cells (n = 237). (C) Representative pictures showing the co-localization of neutrophil (CD66b, green) and T cell markers (CD4 and CD8, red) in the stromal (ST) regions of UCB tissues. (D, E) Consecutive sections were used for the immunohistochemical identification of stromal CD66b+ cells, as well as CD4+ T cells and CD8+ T cells in UCB tissues (n= 57). P < 0.05 was considered significant difference (Spearman’s rank correlation coefficient test). Representative examples of tumors with either low (top) or high (bottom) expression of neutrophil (CD66b), T cells (CD4 and CD8) markers are shown. Scale bar: 100 μm.