TABLE 1.
Pools of in vivo produced bovine embryos used for nanoLC-MS/MS analysis (n = 4 embryos per pool).
| Embryonic stage | Interval of embryo collection after first insemination | Female donor a | Bull semen b | Number of pools |
|---|---|---|---|---|
| 4–6 cells | Days 1.7–3.8 | A, B, C, D | X | 3 |
| 8–12 cells | Days 3.6–3.8 | A, B | X | 4 |
| Morula (>16 cells) | Days 5.7–6.7 | E, F, G | X | 3 |
| Compact morula | Days 5.7–6.8 | E, F, H | X | 4 |
| Blastocyst | Days 6.8–7.5 | H, I, J, K | X, Y, Z | 4 |
a
A total of 9 Holstein cows (called A to I) and 2 Holstein heifers (called J and K) were used as female donors.
b
A total of 3 bulls of proven fertility, one Normand (called X) and two Holstein (called Y and Z), were used for the insemination of female donors.