FIGURE 3.

Characterization of endothelium-dependent relaxations in response to RRBE in coronary artery rings. Intact rings were incubated with (A) the NO synthase inhibitor (L-NA, 100 μM), cyclooxygenase inhibitor (indomethacin, 10 μM), and non-selective potassium channel blocker (TEA, 1 mM), in addition to (B) sGC inhibitor (ODQ, 10 μM) and PI3K inhibitor (wortmannin, 30 nM) for 30 min before the addition of U46619. Results are presented as mean ± SEM of 9–12 experiments. ***p < 0.001 vs Control. Representative and cumulative data of phosphorylation level of eNOS at Ser1177 (C) and Src at Tyr418 (D) in endothelial cells exposed to RRBE as determined by western blot. ***p < 0.001 vs control. **p < 0.01 vs control.