FIGURE 1.

Individual cell isolation in sc-multiomics. Single-cell multiomics techniques differ in when and how, during their workflow, they isolate single cells into individual reactions for further processing. (A) Different stages at which cells are isolated in sc-multiomics. While some sc-multiomics techniques 1) start from the single-cell suspension by first isolating individual cells (using one of the approaches in (B) and only then subject cells individually to further processing, such as enzymatic treatments (top), other techniques 2) subject the single-cell suspension to enzymatic treatment first and only then isolate single cells (bottom). (B) Different technologies for isolating individual cells in sc-multiomics. Well-based methods rely on techniques such as fluorescence-activated cell sorting (FACS) to sort individual cells into unique wells. Valve microfluidics methods treat single cells individually in unique reaction wells on microfluidic chips. Droplet microfluidics encapsulate individual cells into unique barcoded droplets for further processing.