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Use of barcoding at different steps in sc-multiomics. Barcoding of DNA and RNA can be employed at different steps of the processing of single cells, and different labels and techniques can be used at one or more of these steps. A first barcode can be inserted in DNA during tagmentation and in RNA during RT. Then, after pooling of samples and redistribution into wells, one or more ligation-based barcoding is possible. A third barcoding is possible during amplification.
