Mass spectrometry optimized parameters for vancomycin and Turgidosterone 4–7.

Compounds	Mode of analysis	Precursor ion (m/z)	Product ion (m/z)	Declustering potential (V)	Collision energy (V)	Collision cell exit potential (V)	Dwell time (ms)
4	Negative	1029.36	737.4a	−25	−64	−37	50
		1029.36	59b	−25	−130	−7	50
5	Positive	869.48	70.9a	1	95	10	100
		869.48	84.8b	1	75	14	100
6	Negative	1013.34	721.4a	−20	−60	−55	100
		1013.34	59b	−20	−130	−19	100
7	Positive	885.39	293.2a	1	23	8	50
		885.39	413.3b	1	29	20	50
Vancomycin (IS)	Negative	722.81	700.7	−190	−20	−27	50
Vancomycin (IS)	Positive	724.73	83	141	67	8	50

^aQuantifier ion.

^bQualifier ion.