Table 2.
Primary and secondary antibodies used in Western blot.
| Protein | Source (cat. number) | Dilution | Secondary antibody dilution |
|---|---|---|---|
| Mrp2 | Enzo (ALX-801-037-C125) | 1:300 | 1:2,000 |
| Mrp3 | Thermo Fisher (PA5–101482) | 1:1,000 | 1:2,000 |
| Mrp4 | Cell signaling (12857S) | 1:1,000 | 1:2,000 |
| Cyp27a1 | Thermo Fisher (PA5-27946) | 1:1,000 | 1:2,000 |
| Cyp7a1 | Sigma Aldrich (MABD42) | 1:1,000 | 1:2,000 |
| Cyp8b1 | Thermo Fisher (PA5–37088) | 1:1,000 | 1:2,000 |
| Cyp2c70 | MyBioSource (MBS3223844) | 1:1,000 | 1:2,000 |
| Ntcp | Sigma-Aldrich (SAB2108757) | 1:1,000 | 1:2,000 |
| Bsep | Thermo Fisher (PA5-78690) | 1:000 | 1:2,000 |
| p-SAPK/JNK | Cell signaling (4668S) | 1:1,000 | 1:2,000 |
| p-Erk 1/2 (P-P44/42) | Cell signaling (4370S) | 1:1,000 | 1:2,000 |
| RXRa | Cell signaling (5388S) | 1:1,000 | 1:2,000 |
| P65_NFKB | Abcam (ab16502) | 1:1,000 | 1:2,000 |
| Gapdh | Cell signaling (2118) | 1:8,000 | 1:10,000 |