Compounds identified in the dichloromethane of leaves (DCM-L), and stem branches (DCM-S) of M. macroura by using UHPLC-ESI/MS/MSa.
| Peak no. | RT | RRT | MS1−/+ | MS2 | Leaves | Stem branches | Tentative assignment |
|---|---|---|---|---|---|---|---|
| 1 | 2.10 | 0.084 | 153/− | 109 | — | √ | Protocatecheic acid |
| 2 | 2.43 | 0.097 | 339/− | 177 (100%) | 0.95 | — | *Aesculin |
| 3 | 4.84 | 0.194 | 137/− | 93 (100%) | — | 0.51 | Hydroxybenzoic acid |
| 4 | 6.27 | 0.251 | 339/− | 177 175 161,135 109 | √ | — | Morachalcone A |
| 5 | 6.27 | 0.251 | 431/− | 269 (100%) | 0.95 | — | *Apigenin-7-O-β-glucoside |
| 6 | 6.73 | 0.269 | 163/165 | 119 (100%) | — | 0.24 | Coumaric acid |
| 7 | 6.92 | 0.277 | 463/− | 270.7 | 1.85 | — | *Naringenin derivatives |
| 8 | 7.26 | 0.291 | 447/− | 285,256.9, 242.5,198.8 | 0.47 | — | *Luteolin-7-O- glucoside |
| 9 | 7.46 | 0.299 | −/355 | 193 | 0.05 | — | Scopolin |
| 10 | 7.62 | 0.305 | 431/− | 341, 311, 269 | 1.09 | — | Apigenin-8-C-glucoside |
| 11 | 7.92 | 0.317 | 463/− | 301, 257 | 4.09 | — | *Morin-O-β-glucoside |
| 12 | 8.10 | 0.325 | −/449 | 287 | √ | — | Cyanidin-3-O-glucoside |
| 13 | 8.25 | 0.331 | 463/− | 301 (100%) | 7.33 | — | Quercetin-3-O-β-D-glucoside |
| 14 | 8.38 | 0.336 | 463/− | 301 | — | √ | Quercetin-3-O-β-galactoside |
| 15 | 8.96 | 0.359 | 431/− | 269 | 0.01 | — | *Apigenin-7-O- glucoside isomer |
| 16 | 9.24 | 0.370 | 447/− | 285 | 7.33 | — | Kaempferol-3-O-β-glucoside |
| 17 | 9.24 | 0.370 | 447/− | 343, 301 | 0.47 | — | Quercetin-8-C-rhamnoside |
| 18 | 10.95 | 0.439 | −/611 | 303, 328 | 0.80 | — | *Hesperidin |
| 19 | 10.95 | 0.439 | −/610 | 317.5, 256.8, 151.2 | √ | — | *Petunidin dirhamnoside |
| 20 | 11.14 | 0.446 | 447/− | 301 255 | 0.41 | — | Quercetin-3-O-β- rhamnoside |
| 21 | 11.18 | 0.448 | −/397 | 351 | 1.61 | 8.45 | Cerotic acid |
| 22 | 11.34 | 0.454 | 301/− | 139 (100%), 256 | 0.41 | — | Ellagic acid |
| 23 | 11.34 | 0.454 | 355/− | 193.3 (100%) | — | √ | *Ferulic acid-O-glucoside |
| 24 | 11.37 | 0.456 | 431/− | 285 284, 255 227 | — | √ | *Kaempferol-3-O-β- rhamnoside |
| 25 | 12.10 | 0.485 | 301/303 | 179, 151, 121 | 0.41 | — | Quercetin |
| 26 | 12.17 | 0.488 | 301/− | 256,151 107 | — | √ | Morin |
| 27 | 14.06 | 0.563 | 467/469 | 305 | — | 5.18 | *Gallocatechin glycoside |
| 28 | 14.15 | 0.567 | 467/469 | 305 | — | 5.18 | *Epigallocatechin glycoside |
| 29 | 14.39 | 0.577 | 329/− | 287 [M–H–COCH3] | 2.30 | 13.36 | Phloracetophenone-4-O-glucoside |
| 30 | 14.68 | 0.588 | 575/− | — | — | 1.05 | β-Sitosterol-3-O-β-D-glucoside |
| 31 | 16.04 | 0.643 | 467/− | — | 0.27 | — | Lupeol acetate |
| 32 | 17.77 | 0.712 | −/579 | — | 1.78 | — | *Nonatriacontanoic acid |
| 33 | 17.83 | 0.715 | 353/355 | 203 | — | √ | *Epoxybergamottin |
| 34 | 17.83 | 0.715 | 353/355 | 299, 69 | — | 0.62 | Albanin A |
| 35 | 18.38 | 0.737 | 293/295 | 220.8 (100%) | — | 0.01 | *Hydroxy octadecatrienoic acid |
| 36 | 18.87 | 0.756 | 591/− | 287 | √ | — | *Cyanidin cinnamoyl glucuronide |
| 37 | 19.77 | 0.792 | 317/− | — | 0.86 | 1.73 | Unknown |
| 38 | 21.08 | 0.845 | 475/477 | 299.0 | — | 0.67 | *Chrysoeriol-uronic acid |
| 39 | 21.40 | 0.858 | 433/− | 271 | — | √ | *Naringenin-7-O-β-glucoside |
| 40 | 21.49 | 0.861 | 379/381 | 318, 163, 71, 69 | 1.61 | — | *Brassicasterol |
| 41 | 21.99 | 0.881 | 475/477 | 255 | — | 0.67 | Palmitic acid ester |
| 42 | 22.73 | 0.911 | −/683 | 331 | — | √ | *Malvidin diglucoronide |
| 43 | 23.02 | 0.922 | 451/- | 249.8390.5406.7435.1 | 0.80 | 16.23 | *Melissic acid |
| 44 | 23.06 | 0.924 | -/683 | 597, 435, 287 | — | 0.88 | *Cyanidin derivative |
| 45 | 23.24 | 0.931 | 255/− | — | 2.10 | 2.81 | Palmitic acid |
| 46 | 23.47 | 0.941 | 281/− | — | 1.08 | 2.14 | Oleic acid |
| 47 | 23.55 | 0.944 | −/391 | 279, 149 (100%) | 4.49 | 8.30 | *Bis (2-ethyl hexyl) phthalate |
| 48 | 23.72 | 0.951 | −/593 | 287 434, 595 | 9.12 | 8.45 | Cyanidin rutinoside |
| 49 | 23.83 | 0.955 | 349/− | — | 1.08 | 2.14 | *Pentacos-7-ene |
| 50 | 23.86 | 0.956 | 395/− | 281 | — | √ | Oleic acid ester |
| 51 | 24.14 | 0.967 | −/607 | 286 | 0.23 | 2.48 | Australisine A |
| 52 | 24.44 | 0.979 | 283/− | 239 [M–H–COO] | 0.52 | 0.82 | Octadecanoic acid |
| 53 | 24.65 | 0.988 | −/535 | 317, 153 | 6.30 | 6.32 | *Isorhamnetin-3-O-acetyl glucuronide |
| 54 | 24.65 | 0.988 | −/536 | 317, 153 | — | 0.02 | *Petunidin 3-O-acetyl glucuronide |
| 55 | 24.95 | 1.000 | 339/− | 163 (100%) | 4.75 | 8.66 | Euchrenone a7 |
| 56 | 25.29 | 1.013 | −/397 | 254.9 | 3.90 | 2.99 | β-Sitosterol |
| 57 | 25.47 | 1.020 | −/535 | 331 | 6.30 | 6.32 | *Malvidin-3-O-acetylglucuronoide |
| 58 | 25.53 | 1.023 | −/683 | 597(loss of 86), 435 (loss of glucose), 287.7 | 10.15 | 12.42 | *Cyanidin-3-O-malonyl glucoside derivative |
| 59 | 25.84 | 1.035 | −/535 | 287 | 6.99 | 15.18 | *Cyanidin-3-O-malonyl glucoside |
| 60 | 26.34 | 1.055 | 661/663 | — | 0.30 | — | *Lupeol palmitate |
| 61 | 26.59 | 1.065 | −/611 | 287, 449 | 3.90 | 2.99 | *Cyanidin-3-O-hexosyl hexoside |
| 62 | 26.82 | 1.075 | 475/− | 299.8, 429 | — | 0.67 | *Peonidin-3-O-glucuronide |
| 63 | 27.68 | 1.109 | −/398 | 314 | 6.40 | 9.35 | *24-Methylene-ergosta-5-en-3β-Ol |
| 64 | 28.13 | 1.127 | 455/− | 400.5 | — | 8.66 | Oleanolic acid |
a
*Compounds identified for the first time in the family Moraceae. Bold with or without * means compounds isolated as pure metabolites from M. macroura. √ Minor compounds. — Compounds not detected.