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. 2022 Mar 23;18(3):e1010431. doi: 10.1371/journal.ppat.1010431

Fig 5. Mutational analysis of 16E6-induced cell colony competition.

Fig 5

A. 16E6 mutations that interrupt interaction with p53 (F2V, E18K, F47R), NHERF1 (F69A/K72A), or PDZ-domain proteins (Δ150–151) were introduced into red NIKS keratinocytes and set into competition with green NKS keratinocytes as described in Fig 1. 16E6 with a stop codon at amino acid 12 served as the negative control and colony sizes are normalized to that sample. 16E6 with a stop codon at position 55 (E6*, R55-stop) produces 16E6* but cannot produce full length 16E6. The bar graph combines the results of 4 separate normalized experiments. *** signifies P<0.001, n.s. signifies non-significant differences. Fig 4B. Western blot analysis of 16E6 and mutant 16E6 protein expression. E6* is not shown because it is not recognized by the 6G6 monoclonal anti-16E6 antibody.