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Active targeting study of pDNA/DSP-NPs. (A) Uptake of naked Cy5-pDNA and Cy5-pDNA-NPs by RAW264.7 cells with LPS activation. Scale bar, 75 μm. (B) Western blotting (WB) assay of SPARC expression in normal and CIA rats. (C) The expression of SPARC was quantitatively analyzed by ImageJ. Results were presented as mean ± SD (n = 3). (D) Immunohistochemical (IHC) staining assay of SPARC expression in normal and CIA rats.
