| Strain, strain background (S. cerevisiae) |
BY4741 |
Baker Brachmann et al., 1998
|
– |
BY parent background |
| Strain, strain background (S. cerevisiae) |
RM11-1a |
Brem et al., 2002
|
– |
RM parent background |
| Strain, strain background (S. cerevisiae) |
YAN696 |
This paper |
– |
BY parent |
| Strain, strain background (S. cerevisiae) |
YAN695 |
This paper |
– |
RM parent |
| Strain, strain background (S. cerevisiae) |
BB-QTL F1 strain library |
This paper |
– |
100,000 strains of barcoded BYxRM F1 produced, genotyped and characterized in this paper |
| Strain, strain background (E. coli) |
BL21(DE3) |
NEB |
NEB:C2527I |
Zymolyase and barcoded Tn-5 expression system |
| Recombinant DNA reagent |
pAN216a pGAL1-HO pSTE2-HIS3 pSTE3 LEU2 (plasmid) |
This paper |
– |
For MAT-type switching |
| Recombinant DNA reagent |
pAN3H5a 1/2URA3 KanP1 ccdB LoxPR (plasmid) |
This paper |
– |
Type one barcoding plasmid, without barcode library |
| Recombinant DNA reagent |
pAN3H5a LoxPL ccdB HygP1 2/2URA3 (plasmid) |
This paper |
– |
Type two barcoding plasmid, without barcode library |
| Sequence-based reagent |
Custom Tn5 adapter oligos |
This paper |
Tn5-L and -R |
See Materials and methods |
| Sequence-based reagent |
Custom sequencing adapter primers |
This paper |
P5mod and P7mod |
See Materials and methods |
| Sequence-based reagent |
Custom barcode amplification primers |
This paper |
P1 and P2 |
See Materials and methods |
| Sequence-based reagent |
Custom sequencing primers for Illumina |
This paper |
Custom_read_1 through 4 |
See Materials and methods |
| Software, algorithm |
Custom code for genotype inference |
This paper |
– |
See code repository |
| Software, algorithm |
Custom code for phenotype inference |
This paper |
– |
See code repository |
| Software, algorithm |
Custom code for compressed sensing |
This paper |
– |
See code repository |
| Software, algorithm |
Custom code for qtl inference |
This paper |
– |
See code repository |
