Table 1. Each parameter varied in the RD model to reproduce in vitro experimental kinetics, along with the optimal values we found.

Other energies, geometries, and concentrations were fixed by experimental data.

Parameters	Explanations	Optimal values
ρ AP	AP-2 density on membrane	0.009 nm-2
k AP−CLA	AP-2 & clathrin binding rate	0.0012 μM-1s-1
k CLA−CLA	Clathrin & clathrin binding rate without AP-2	0.083 μM-1s-1
ΔG coop	Cooperative energy from AP-2 binding	-2.4 kBT
ΔG strain	Strain energy for closed polygons	6.9 kBT
h	Length-scale from dimensional reduction	30 nm