Table 1.
Target gene | Amplicon (bp) | Primers (5′-3′) | T m | Reference |
---|---|---|---|---|
InvA | 423 | F: TCGTGACTCGCGTAAATGGCGAA R: GCAGGCGCACGCCATAATCAATA |
63°C | [15] |
| ||||
IE | 316 | F: AGTGCCATACTTTTAATGAC R: ACTATGTCGATACGGTGGG |
55°C | [16] |
| ||||
gyrA | 610 | F: CGAGAGAAATTACACCGGTCA R: AGCCCTTCAATGCTGATGTC |
55°C | [19] |
| ||||
blaTEM-1 | 643 | F: CAGCGGTAAGATCCT TGAGA R: ACTCGCCGTCGTGTAGATAA |
54°C | [20] |
| ||||
tetB | 659 | F: TTGGTTAGGGGCAAGTTTTG R: GTAATGGGCCAATAACACCG |
57°C | [21] |
| ||||
Cas1 | 892 | F: CCAGTGATTCAGGTTCCGGT R: GTGACGTTCGTACCGCTCAA |
55°C | This study |
| ||||
Cas2 | 262 | F: AACCAAACGCAGTCCATCCA R: TATGGTGGTTGTGGTCACGG |
55°C | This study |
| ||||
Cas3 | 692 | F: GCAAAGTCCGTCACCACAAT R: GATTTAGCGCCGGTGGATTT |
55°C | This study |
| ||||
Cas3∗ | 201 | F: GGGATAGACATAGGCGCTGT R: GATTTAGCGCCGGTGGATTT |
55°C | This study |