Fig. 1.

Defects in spermatogenesis in Asz1^–/– mice. (A) Expression of Asz1 in gonocytes of 1 dpp Asz1^+/– and Asz1^–/– mice. (B) Western blotting of testes from 3-week-old wild-type (left) and Asz1^–/– mice (right) and livers from 3-week-old Asz1^+/– mice (negative control, middle). Western blot analysis were performed using three biologically independent samples, however, three lanes for each sample are technical replicates in this gel image. (C) Testes from 6-week-old wild-type (left), Asz1^+/– (middle), and Asz1^–/– mice (right). The ruler scale is 1 mm wide. (D) Histological analysis of testes from 2-, 4-, and 6-week-old Asz1^+/– (top) and Asz1^–/– mice (bottom). An arrowhead indicates an elongated spermatid. Scale bars indicate 100 μm.