Fig. 2.
Failure of synapsis in spermatocytes and of nuclear localization of MIWI2 in gonocytes of Asz1–/– mice. (A) Immunofluorescence of the synaptonemal complex in spermatocytes from the testes of 3-week-old wild-type (left) and Asz1–/– (right) mice. SCP1 and SCP3 were labeled red and green, respectively. Scale bars indicate 10 μm. (B) Localization of MIWI2 (red) in gonocytes from testes at E17.5 in wild-type (left) and Asz1–/– (right) mice. Nuclei (blue) were counterstained with DAPI. Scale bars indicate 10 μm. (C) Expression of LINE1 open reading frame1 protein (L1ORF1p, green) in gonocytes from testes at E17.5 in wild-type (left) and Asz1–/– (right) fetuses. Nuclei (blue) were counterstained with DAPI. Scale bars indicate 10 μm.