TABLE 1.
Oligonucleotides used
| Designation | Sequence (5′ to 3′)a | 5′ coordinate | Accession no. |
|---|---|---|---|
| A | CCGAGAAGCTGGTCAAAC | 1703 (+); 18S | M60302 |
| B | ACCAAGAGATCCGTTGTTG | 26 (−); 5.8S | X71088 |
| C | GCATGCCTGTTTGAGCGTC | 136 (+); 5.8S | X71088 |
| D | CAGCGGGTAGTCCTACCTG | 72 (−); 25S | X70659 |
| F | ATTTAGGTGACACTATAGAATTAAAACATAGCATTGTGATG | 2158 (+); 25S | X70659 |
| H | CCGAAGCTCCCACTTATTC | 2495 (−); 25S | X70659 |
| J | CAATGTGATTTCTGCCCAGTG | 2201 (+); 25S | X70659 |
| K | CACAATGTCAAACTAGAGTCAAG | 2457 (−); 25S | X70659 |
| L | CATTGCTCCAAGAAATCGC | 379 (−); Ca.LSU intron | X74272 |
| 10816 | GGTGATGGTGTTACTCACG | 2150 (+); actin | X16377 |
| 10473 | GCTTCCAAACCTAAATCAGC | 2496 (−); actin | X16377 |
| Oligo(dT) | T18 |
a
Underlined sequence in primer F is a promoter sequence from bacteriophage SP6; the 5′ coordinate shown is for nucleotide 19 of this sequence.