Fig. 4. DNMT3A^R882/IDH^mut double mutant AMLs display an attenuated focal hypermethylation phenotype.

A Locus heatmap of mean methylation at IDH^mut DMRs (rows) in IDH1 or IDH2 mutant, DNMT3A^R882/IDH^mut double mutant, and DNMT3A^R882 AMLs, and CD34+ cells. B Distribution of IDH^mut-specific DMR methylation levels by AML subtype. C Example IDH^mut-specific DMR locus within the ETV6 gene demonstrating an intermediate methylation phenotype of double mutant samples with respect to IDH^mut and DNMT3A^R882 mutant AMLs. D Methylation locus heatmap of average subtype methylation across DNMT3A^R882 DMRs called vs. CD34+ cells in IDH^mut, DNMT3A^R882/IDH^mut double mutant, and DNMT3A^R882 AMLs, and CD34+ cells. E Distribution of DNMT3A^R882 DMR methylation levels by AML subtype. F Example DNMT3A^R882 DMR locus within the MLLT1 gene, demonstrating the hypomethylation phenotype of DNMT3A^R882 mutant samples with respect to IDH^mut and DNMT3A^R882/IDH^mut double mutant AML samples.