Fig. 1. Setd5 deficiency causes phenotypic HSPC expansion.

A FACS analysis of T, B, and myeloid cells frequency in PB cells; n = 5. B Relative frequency of immature cells (Lin^– and c-Kit⁺) in BM. Lin: lineage cocktail; n = 5. C The absolute cell number of HPC (LK: Lin^–c-Kit⁺Sca-1^–), CMP (Lin^–c-Kit⁺Sca1^–CD34⁺CD16/32^low), GMP (Lin^–c-Kit⁺Sca1^–CD34⁺CD16/32^high) and MEP (Lin^–c-Kit⁺Sca1^–CD34^–CD16/32^low) populations in Setd5^fl/fl and Setd5^CKO mice; n = 5. D–F FACS analysis of LSK⁺s (Lin^–Sca1⁺c-Kit⁺) and SLAM-HSCs (Lin^–Sca1⁺c-Kit⁺CD150⁺CD48^–) and absolute cell number in BM. SLAM-MPP: Lin^–Sca1⁺c-Kit⁺CD150^–CD48^–, HPC1: Lin^–Sca1⁺c-Kit⁺CD150^–CD48⁺, HPC2: Lin^–Sca1⁺c-Kit⁺CD150⁺CD48⁺; n = 5. G Representative FACS profiles of Ki67 staining of SLAM-HSCs. H, I The frequencies of G0, G1, S/G2/M phases in SLAM-HSCs and LSK⁺s are shown; n = 4. J, K Apoptosis analysis of HSPCs in Setd5^fl/fl and Setd5^CKO mice, LT-HSC: Lin^–Sca1⁺c-Kit⁺CD34^–Flt3^low, ST-HSC: Lin^–Sca1⁺c-Kit⁺CD34⁺Flt3^low, MPP: Lin^–Sca1⁺c-Kit⁺CD34⁺Flt3⁺; n = 5. Data are represented as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001.