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. 2021 Dec 1;36(4):1111–1122. doi: 10.1038/s41375-021-01481-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — A–E Serial competitive repopulation assays with Setd5^fl/fl and Setd5^CKO LSK⁺ cells. Strategy for serial competitive repopulation assays (A), BMT: BM transplantation. Quantification of donor-derived (CD45.2) cells (B), donor-derived myeloid (M: CD11b⁺) cells (C), donor-derived T (CD3⁺) cells (D) and B (B220⁺) cells (E) in PB at indicated time points in primary (n = 9) and secondary (n = 10) recipients. F Proportion of each lineage in donor derived PB cells; n = 5. G Donor contribution of indicated cell populations in BM cells of primary and secondary recipient mice at indicated times; n = 5. H Poisson statistical analysis from the limiting dilution assay. LTR-HSCs: long-term repopulating (LTR)-HSC. Symbols represent the percentage of negative mice for each dose of cells. Solid lines indicate the best-fit linear model for each dosage. Dotted lines represent 95% confidence intervals. I Frequencies of functional HSCs were calculated according to Poisson statistics. Data are represented as Mean ± SEM; * P < 0.05, ** P < 0.01, *** P < 0.001.