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. 2021 Dec 1;36(4):1111–1122. doi: 10.1038/s41375-021-01481-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — A Density plots of SETD5 (blue) and PAF1 (green) normalized ChIP-seq signals. B, C Occupancy of total Pol II on all genes and DEGs (Fold change>1.5 and p adj value<0.05), ChIP normalization was implemented by integrating Spike-in Chromatin. D, E Density plot of all genes and DEGs with reduced average Pol II pausing index in Setd5^CKO. F IGV tracks comparing occupancy of Pol II within Cdk1 locus. G, H ChIP-qPCR of Pol II and Pol II Ser2P at the promoters of indicated genes in c-Kit⁺ cells from control or Setd5^CKO mice; n = 2. I ChIP-qPCR analysis of PAF1 at the promoters of indicated genes in c-Kit⁺ cells; n = 2. J The H3K9 dimethylation levels at Cdc20 and Cenpe promoters; Gapdh as an internal control; n = 2. Mean ± SEM; *P < 0.05, **P < 0.01, *** P < 0.001.