Fig. 4. Genetic depletion of MCL-1 sensitizes ALL cells to venetoclax.

Knockout of MCL-1 was performed using CRISPR/Cas9 gene editing in NALM-6 and RCH-ACV. A Successful knockout of MCL-1 was confirmed by western blot. B MCL-1 knockout cells and their corresponding controls were exposed to increasing concentrations of venetoclax followed by assessment of cell death by flowcytometry by forward/side scatter criteria in order to determine half maximal effective concentrations. N = 3 independent experiments in triplicates. Differences of MCL-1 knockout cell lines and corresponding control cells were determined by extra-sum-of-squares F test. F value; degrees of freedom numerator (DFn) and denominator (DFd); p, significance.